Leica STELLARIS 8 Confocal Microscope New generation

Description

Leica STELLARIS 8 Confocal Microscope Is New generation high-end inverted laser scanning confocal microscope. Versatile system equipped with CW 405nm laser, new generation white light laser (range 440-790nm), and discrete 488nm laser.

The system is equipped with the new high-sensitive time-resolved hybrid detectors (2× HyD S, 2× HyD X, 1× HyD R) and a PMT detector for transmitted light. Full control on lifetime information are available thought TauSense and FAst Lifetime CONtrast (FALCON) modules and advanced dynamic information can be obtained thought fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS).

Leica STELLARIS 8 is a forward-looking system, offering all the benefits of the STELLARIS 5 core system plus added functionality thanks to an extended spectrum White Light Laser (WLL) and specialized detector options of the Power HyD family. This allows you to expand the range of confocal applications in your research.

Leica STELLARIS 8 8 can be combined with all Leica Microsystems modalities, including FAst Lifetime CONtrast (FALCON), multi-photon imaging with Deep In Vivo Explorer (DIVE), super-resolution Tau-STED, Digital Light Sheet (DLS) and Coherent Anti-Stokes Raman Scattering. STELLARIS 8 new features maximize the potential of these modalities and give you the power and potential to set new standards for research.

Leica STELLARIS 8 Features

• Power to see more

The synergy between the Power HyD detector family, optimized beam path, and White Light Lasers (WLL) empowers you to obtain more accurate and reliable data to test your hypothesis with precision.

• Potential to discover more

TauSense gives instant access to lifetime-based information, delivering additional insights to your experiments.

• Productivity to do more

The ImageCompass user interface enables you to acquire images with just a few clicks, even when doing complex experiments.

• The POWER to see more

The Power HyD detector family provides higher photon detection efficiency (PDE)* extremely low dark noise, and sensitive spectral detection from 410 to 850 nm.

• Enhanced image quality: STELLARIS provides an ideal combination of brightness, resolution and contrast.
• Complete spectral freedom: Our next-generation White Light Lasers allow you to simultaneously use up to 8 single excitation lines from across the spectrum. Image more fluorophore combinations than with any other confocal platform and use more labels in parallel, expanding your options into the NIR range.
• Gentle Live Cell Imaging: Preserve your sample’s integrity and image for longer periods of time through efficient signal acquisition at the lowest required levels of illumination

*2x higher Photon Detection Efficiency (PDE) compared to conventional multi-alkali Photomultiplier Tubes (PMT) and 3x higher in the extended red range.

• The POTENTIAL to discover more

STELLARIS unique TauSense technology allows you to extract an extra layer of information from every sample and increase the scientific impact of your research. TauSense is comprised of application-oriented imaging tools based on fluorescence lifetime that you can use to explore the function of molecules within the cellular context.

• TauContrast provides immediate access to functional information, such as metabolic status, pH, and ion concentration.
• TauGating improves the quality of your images by removing unwanted fluorescence contributions.
• TauSeparation helps you expand the combination of fluorescent signals in your experiment beyond the spectral options.
• TauInteraction provides straightforward detection and quantification of molecular interactions (e.g. protein-protein interaction).

• The PRODUCTIVITY to do more

Streamlined setup and acquisition: ImageCompass, the STELLARIS smart user interface, provides users with an easy and intuitive way to set up even the most complex experiments with just a few clicks.

• Simply fast: Superb image quality in real time and at full speed with the LIGHTNING super-resolution, Dynamic Signal Enhancement (DSE), and the powerful AI feature AiviaMotion.
• Intuitive User Interface: ImageCompass guides you from the experiment setup through acquisition.
• Optimize your experiment: Tools like LAS X Navigator are seamlessly integrated for straightforward imaging.

• Analyze your images for hidden insights

Aivia and LAS X, advanced image analysis software solutions from Leica Microsystems empower you to take your microscopy-based research to the next level.

By combining STELLARIS with Leica’s AI-driven image analysis software Aivia, you can gain meaningful insights from your microscopy imaging data through a wide range of machine learning and deep learning algorithms.

Leica STELLARIS 8 specification

• Dimensions(WxDxH) 335 x 100 x 175 cm (11 x 3.3 x 5.9 in.)
• QuantityEA
• SpeedField-of-view scanner –

• Maximal frame rate: 512 x 512 10 fps
• Maximal frame rate: 512 x 16 131 fps

Resonant scanner 8kHz –

• Maximal frame rate: 512 x 512 28 fps
• Maximal frame rate: 512 x 16 290 fps

Resonant scanner 12kHz –

• Maximal frame rate: 512 x 512 40 fps
• Maximal frame rate: 512 x 16 428 fps

• ApplicationsLive Cell Imaging, 3D Imaging, FRET, FRAP, confocal imaging, fluorescence, multicolor, STED, Tau STED, Super Resolution, Nanoscopy, Multiphoton, FLIM, Lightsheet
• SensitivityPOWER HyD S:

• Spectral sensitivity range: 410 to 850nm
• Typical photon detection efficiency (PDE): > 58% @ 500nm

POWER HyD X:

• Spectral sensitivity range: 410 to 750nm
• Typical photon detection efficiency (PDE): > 46% @ 500nm

POWER HyD R:

• Spectral sensitivity range: 410 to 830 nm, tunable
• Typical photon detection efficiency (PDE): > 26 % @ 635nm

• DetectorPower HyD S, Power HyD X, Power HyD R
• ResolutionUp to 120 nm (Lateral resolution)
  Up to 200 nm (Axial resolution)
• Wavelength(s)410 to850 nm, tunable
• ObjectivesUV/405 correction: Unified concept with CS2 optics
• LasersContinuous Wave Lasers: 355 nm, 405 nm, 448 nm, 488 nm, 514 nm, 561 nm, 638 nm, 730 nm
  Pulsed Lasers: 485–790 nm

Reference

• Manufacturer information
• Brochure