ZEISS LSM 900 with Airyscan 2 Confocal Microscopes

Description

The ZEISS LSM 900 with Airyscan 2 Confocal Microscopes setup is configured for confocal fluorescent imaging of fixed samples. It is mounted on a fully motorized AxioObserver Z1 inverted microscope stand and features hardware-based Z drift compensation and a Piezo stage for precise z-stepping. The stage is also motorized in X,Y to mark positions or acquire images of areas larger than the field of view through tiling of individual fields of view.

The Zeiss LSM 900 for confocal 4D imaging has been designed with high sensitivity and spectral flexibility. The Airyscan 2 with its Multiplex mode allows to profit from smart detection schemes and parallel (=faster) pixel acquisition.

Zeiss LSM900 provides a convenient solution for most of your imaging needs. Additionally, the AI Sample Finder and advanced Navigation and Tiles module make it very easy to find regions of interest and set up your imaging experiments.

This system has 2 independent sensitive GaAsP detectors as well as the Airyscan detector which can be used as a normal detector. The Airyscan module allows also image acquisition either in super-resolution mode with lateral resolution below 120nm or in one of many multiplex modes to increase image acquisition speed.

ZEISS LSM 900 with Airyscan 2 Features

• The Airyscan 2 detector provides sub-diffraction limited imaging down to 120 nm (XY) and 350 nm (Z) resolution.
• The software comes with an Airyscan Joint Deconvolution module that can improve resolution to 90 nm (XY) and 270 nm (Z).
• The new AI Sample Finder can identify areas of interest and setup scan areas automatically.
• The software, Zen Blue 3.6, offers greatly improved tile scan functionality.
• An Airyscan Multiplex 4Y option allows confocal imaging at over 3 frames per second.

LSM 900 is ideally suited for live imaging due to the attached incubation chamber, allowing full control of temperature, humidity and/ CO2.

The system comprises 6 objectives (2.5x,10x,20x,40x (oil), 63x (multi-immersion), 63x (oil) for Airyscan) and 4 visible laser lines (405/488/561/638 nm).

The Zeiss LSM 900 comes with AI Sample Finder for automated sample identification for efficient imaging. The system includes 405nm, 488nm, 561nm, and 640nm solid-state lasers, Airyscan 2 for super resolution imaging, 2x GaAsP-PMTs for confocal imaging, cohesive contrast, as well as temperature/CO2/humidity control stage though a stage-top incubator and an incubated chamber for the microscope.

ZEISS LSM 900 with Airyscan 2 Specifications

Excitation

• Colibri 5 (RGB-UV) Fluorescence LED
• CW 405nm diode laser (5mW)
• CW 488nm diode laser (10mW)
• CW 561nm diode laser (10mW)
• CW 640nm diode laser (5mW)

Filter sets

The microscope and scanhead are equipped with dedicated filters and variable beam splitter dichroics for optimal detection by the three detector units.

Detection

The microscope is equipped with the following detectors:

• 2x GaAsP PMT detectors.
• Airyscan 2 (type 63x) 32 channel GaAsP array for super-resolution imaging.

Objective lenses

The microscope is equipped with a variety of low- and high-magnification and NA objectives:

Objectives listed in bold are permanently available. Other listed objective lenses can be provided on demand.

One position is permanently occupied by the AI Sample Finder deflecting mirror.

Reference

ZEISS LSM 900  Brochure

Manufacturer Information